Journal: PLoS ONE

Article Title: Translational Regulation of GPx-1 and GPx-4 by the mTOR Pathway

doi: 10.1371/journal.pone.0093472

Figure Lengend Snippet: The effect of imatinib on GPx enzyme activity (A) and GPx-1 protein (B) in MEG-01, MDA-MB-231 and GM10832 is shown. GPx activity was increased 2-fold, and protein increased 4-fold ( P <0.01) by 300 nM imatinib treatment in MEG-01. GPx-1 activity and protein levels did not change following imatinib treatment of MDA-MB-231 (500 nM imatinib) and GM10832 (300 nM). Data presented in A is the result of three independent experiments * = P <0.001. Error bars indicate S.D.

Article Snippet: The GM10832 human immortal B lymphocyte cell line was obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository of the Coriell Institute (Camden, NJ).

Techniques: Activity Assay

Journal: PLoS ONE

Article Title: Translational Regulation of GPx-1 and GPx-4 by the mTOR Pathway

doi: 10.1371/journal.pone.0093472

Figure Lengend Snippet: The effect of rapamycin on GPx-1 activity (A) and protein levels (B) in KU812a, MEG-01 GM10832 or MDA-MB-231 is shown. The effect of rapamycin on steady-state GPx-1 transcript levels as determined by RT-qPCR and normalization of GPx-1 Ct values to Ct values for 18s RNA (C). Rapamycin significantly increased protein respectively 3-fold and 1.3-fold in KU812a and MEG-01 ( P = 0.05). Steady state transcript levels for GPx-1 were unaffected by treatment with rapamycin in KU812a. Data shown is representative of three independent experiments. * = P <0.001, ** = P <0.01. † = P <0.05. Error bars indicate S.D.

Article Snippet: The GM10832 human immortal B lymphocyte cell line was obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository of the Coriell Institute (Camden, NJ).

Techniques: Activity Assay, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Translational Regulation of GPx-1 and GPx-4 by the mTOR Pathway

doi: 10.1371/journal.pone.0093472

Figure Lengend Snippet: The effect of 1 ng/mL rapamycin on GPx-4 and MnSOD and pS6 protein levels in KU812a, MEG-01, GM10832 and MDA-MB-231 is shown. GPx-4 by 3-day 1 ng/mL rapamycin treatment in KU812a and MEG-01 ( P > 0.2), but was increased 3-fold in GM10832 ( P = 0.05) and 6-fold in MDA-MB-231 cells ( P = 0.02). The disappearance of pS6 signal following rapamycin treatment indicates inhibition of mTOR. Data shown is representative of three independent experiments.

Article Snippet: The GM10832 human immortal B lymphocyte cell line was obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository of the Coriell Institute (Camden, NJ).

Techniques: Inhibition

Journal: PLoS ONE

Article Title: Effects of Novel Isoform-Selective Phosphoinositide 3-Kinase Inhibitors on Natural Killer Cell Function

doi: 10.1371/journal.pone.0099486

Figure Lengend Snippet: CCRF-CEM, human acute T lymphocytic leukemia cell line was labeled with calcein AM, incubated with mouse anti-human CD4 mAb, and then co-cultured with NK cells at 10∶1 E:T ratio in the presence of indicated inhibitors for 2 h. Culture supernatants were collected and calcein fluorescence was measured and analyzed as in . The values are presented as the means ± SEM from three independent experiments. * p <0.05 and ** p <0.01, as determined by the unpaired Student's t test using Sigma Plot 10 software and as compared to the vehicle control group.

Article Snippet: CCRF-CEM, human acute T lymphocytic leukemia cell line was kindly provided by Dr. Matthew Janes (Intellikine, La Jolla, CA).

Techniques: Labeling, Incubation, Cell Culture, Fluorescence, Software